Updated on 2024/02/21

写真a

 
YANO Shigekazu
 
Title
Assistant Professor

Research Areas

  • Life Science / Applied microbiology

Academic Society Affiliations

  • The Japanese Society of Applied Glycoscience

  • The Society of Iodine Science

  • Japan Oil Chemists' Society

 

Research Career

  • Studies on Basidiomycete Cell-wall Lytic Enzymes, 2000.04 -

Papers

  • Addition of α-1,3-glucan-binding domains to α-1,3-glucanase Agn1p from  Schizosaccharomyces pombe enhances hydrolytic activity of insoluble α-1,3-glucan., The Journal of general and applied microbiology, advpub(0) , 2024.02

    Horaguchi Y, Yokomichi M, Takahashi M, Xu F, Konno H, Makabe K, Yano S

    Single Author

  • α‑1,3‑Glucanase from the gram‑negative bacterium Flavobacterium sp. EK‑14 hydrolyzes fungal cell wall α‑1,3‑glucan, Scientific Reports, 13 21420, 2023.12

    Masaki Takahashi, Shigekazu Yano, Yui Horaguchi, Yuitsu Otsuka, Wasana Suyotha, Koki Makabe, Hiroyuki Konno, Susumu Kokeguchi

    Multiple Authorship (Including Foreigners)

  • Structure-activity relationship on selective antibacterial activity of nonionic surfactants, JOURNAL OF SURFACTANTS AND DETERGENTS, 26(6) 789-795, 2023.11

    Tanaka, M; Kurose, M; Yano, S; Tanaka, S; Gotoh, N; Nonomura, Y

    Single Author

  • Sulfonium-cation-containing aggregation-induced emission block copolymers: self-assembly, multicolor emission, and detection for DNA polyplexes, European Polymer Journal, 197 112359, 2023.10

    Shotaro Nobe, Kanako Yamamoto, Hirotsugu Miyakawa, Shigekazu Yano, Hiroyuki Furusawa, Hideharu Mori

    Multiple Authorship (Only Japanese)

  • Heterologous expression of alpha-1,3-glucanase Agn1p from Schizosaccharomyces pombe, and efficient production of nigero-oligosaccharides by enzymatic hydrolysis from solubilized alpha-1,3;1,6-glucan, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 87(10) 1219-1228, 2023.07

    Horaguchi Yui, Takahashi Masaki, Takamatsu Keigo, Konno Hiroyuki, Makabe Koki, Yano Shigekazu

    Multiple Authorship (Only Japanese)

  • Enzyme immobilization on α-1,3-glucan: development of flow reactor with fusion protein of α-1,3-glucan binding domains and histamine dehydrogenase., The Journal of general and applied microbiology, advpub(0) , 2023.05

    Nagahashi Y, Hasegawa K, Takagi K, Yano S

    Multiple Authorship (Only Japanese)

  • Introduction of Cation Species to Starch-reinforced Calcium Phosphate Bone Paste, Journal of the Japan Society of Powder and Powder Metallurgy, 70(5) 248-255, 2023.05

    KUWASHIMA Keisuke, YANO Shigekazu, KAWAI Takahiro

    Multiple Authorship (Only Japanese)

  • Nigero-oligosaccharide production by enzymatic hydrolysis from alkaline-pretreated α-1,3-glucan., Journal of bioscience and bioengineering, 135(3) 182-189, 2023.03

    Horaguchi Y, Yano S, Takamatsu K, Otsuka Y, Suyotha W, Wakayama M, Konno H

    Multiple Authorship (Including Foreigners)

  • Cleavage of α-1,4-Glycosidic Linkages by the Glycosylphosphatidylinositol-Anchored α-Amylase AgtA Decreases the Molecular Weight of Cell Wall α-1,3-Glucan in Aspergillus oryzae, Frontiers in Fungal Biology, 3 10.3389/ffunb.2022.1061841, 2023.01

    Ami Koizumi, Ken Miyazawa, Makoto Ogata, Yuzuru Takahashi, Shigekazu Yano, Akira Yoshimi, Motoaki Sano, Masafumi Hidaka, Takanori Nihira, Hiroyuki Nakai, Satoshi Kimura, Tadahisa Iwata, Keietsu Abe

    Single Author

  • Structure–activity relationship on selective antibacterial activity of nonionic surfactants, Journal of Surfactants and Detergents, 2023

    Mayu Tanaka, Mina Kurose, Shigekazu Yano, Seiya Tanaka, Naohiro Gotoh, Yoshimune Nonomur

    Multiple Authorship (Only Japanese)

  • Antimicrobial Activity of Polysorbate 80-iodine Complex: Polysorbate 80 Firmly Retains Iodine., Journal of oleo science, 71(11) 1639-1645, 2022.10

    Yano S, Terada S, Fukushi M, Otsuka Y, Mizunuma S, Asakura S, Kawai T, Konno H, Endo M, Nonomura Y

    Single Author

  • Construction of a fusion protein consisting of α-1,3-glucan-binding domains and tetrameric red fluorescent protein, which is involved in the aggregation of α-1,3-glucan and inhibition of fungal biofilm formation, Journal of Bioscience and Bioengineering, 133(6) 524-532, 2022.06

    Yuitsu Otsuka, Kai Sasaki, Wasana Suyotha, Hiroyuki Hurusawa, Ken Miyazawa, Hiroyuki Konno, Shigekazu Yano

    Multiple Authorship (Including Foreigners)

  • GH-16 type β-1,3-glucanase from Lysobacter sp. MK9-1 enhances antifungal activity of GH-19 type chitinase, and its glucan-binding domain binds to fungal cell-wall, Journal of Applied Glycoscience, advpub(0) , 2022.04

    Otsuka Yuitsu, Sato Koki, Yano Shigekazu, Kanno Haruki, Suyotha Wasana, Konno Hiroyuki, Makabe Koki, Taira Toki

    Single Author

  • A Glycosylphosphatidylinositol-Anchored α-Amylase Encoded by amyD Contributes to a Decrease in the Molecular Mass of Cell Wall α-1,3-Glucan in Aspergillus nidulans, Frontiers in Fungal Biology, 2 , 2022.01

    Ken Miyazawa, Takaaki Yamashita, Ayumu Takeuchi, Yuka Kamachi, Akira Yoshimi, Yuto Tashiro, Ami Koizumi, Makoto Ogata, Shigekazu Yano, Shin Kasahara, Motoaki Sano, Youhei Yamagata, Tasuku Nakajima, Ke...

    Single Author

  • Highly esthetic, deodorant, and antibacterial tile with natural zeolite set via a blast furnace slag-based geopolymer, Journal of the Ceramic Society of Japan, 130(1) 100-106, 2022.01

    KAWAI Takahiro, KOBAYASHI Tatsuya, SASAKI Keiko, NISHIDA Hiroshi, FUJIWARA Wataru, YANO Shigekazu

    Single Author

  • Optimization of protease production by Bacillus cereus HMRSC30 for simultaneous extraction of chitin from shrimp shell with value-added recovered products., Environmental science and pollution research international, 2021.11

    Cahyaningtyas HAA, Suyotha W, Cheirsilp B, Prihanto AA, Yano S, Wakayama M

    Single Author

  • The Selective Antibacterial Activity of the Mixed Systems Containing Myristic Acid against Staphylococci(和訳中), Journal of Oleo Science, 70(9) 1239-1246, 2021.09

    Okukawa Minako, Yoshizaki Yuika, Yano Shigekazu, Nonomura Yoshimune

    Single Author

  • Functional analysis of α-1,3-glucanase domain structure from Streptomyces thermodiastaticus HF3-3(和訳中), The Journal of General and Applied Microbiology, 67(3) 85-91, 2021.06

    Panti Niphawan, Cherdvorapong Vipavee, Itoh Takafumi, Hibi Takao, Suyotha Wassana, Yano Shigekazu, Wakayama Mamoru

    Single Author

  • Cloning, expression, and characterization of a GH 19-type chitinase with antifungal activity from Lysobacter sp. MK9-1., Journal of bioscience and bioengineering, 131(4) 348-355, 2021.04

    Shigekazu Yano, Haruki Kanno, Haruna Tsuhako, Sonoka Ogasawara, Wasana Suyotha, Hiroyuki Konno, Koki Makabe, Keiko Uechi, Toki Taira

    Multiple Authorship (Including Foreigners)

  • Antibacterial Activity of 1,2-Alkanediol against Staphylococcus aureus and Staphylococcus epidermidis , Journal of Oleo Science, 68(8) 759-763, 2019.08

    Okukawa Minako, Watanabe Takamasa, Miura Maki, Konno Hiroyuki, Yano Shigekazu, Nonomura Yoshimune

    Multiple Authorship (Only Japanese)

  • Deletion of uncharacterized domain from α-1,3-glucanase of Bacillus circulans KA-304 enhances heterologous enzyme production in Escherichia coli, The Journal of General and Applied Microbiology, 64(5) 212-220, 2018.11

    Shigekazu Yano, Wasana Suyotha, Sumika Zanma, Hiroyuki Konno, Vipavee Cherdvorapong, Mamoru Wakayama

    Multiple Authorship (Including Foreigners)

  • A novel thermostable α-1,3-glucanase from Streptomyces thermodiastaticus HF 3-3, The Journal of General and Applied Microbiology, 63(5) 296-304, 2017.11

    Wasana Suyotha, Hidehisa Fujiki, Vipavee Cherdvorapong, Kazuyoshi Takagi, Shigekazu Yano, Mamoru Wakayama

    Multiple Authorship (Including Foreigners)

  • Synthesis and evaluation of phenylisoserine derivatives for the SARSCoV3CL protease inhibitor, Bioorganic & Medicinal Chemistry Letters, 27(12) 2746-2751, 2017.06

    Hiroyuki Konno Takumi Onuma, Ikumi Nitanai, Masaki Wakabayashi, Shigekazu Yano, Kenta Teruya, Kenichi Akaji

    Multiple Authorship (Only Japanese)

  • 真菌細胞壁を分解する細菌型α-1,3-グルカナーゼの特性, JSM Mycotoxins, 66(1) 37-43, 2016.01

    矢野 成和, 若山 守

    Multiple Authorship (Including Foreigners)

  • Effective treatment for suppression of acrylamide formation in fried potato chips using L-asparaginase from Bacillus subtilis, 3 Biotech, 5(5) 783-789, 2015.10

    Yohei Onishi, Asep A. Prihanto, Shigekazu Yano, Kazuyoshi Takagi, Midori Umekawa, Mamoru Wakayama

    Multiple Authorship (Including Foreigners)

  • Structure activity relationship study of burkholdine analogues toward simple antifungal agents, Bioorganic & Medicinal Chemistry Letters, 25(16) 199-202, 2015.08

    Hiroyuki Konno, Kanako Abumi, Yasuhiro Sasaki, ShigekazuYano, Kazuto Nosaka

    Multiple Authorship (Only Japanese)

  • Characterization of α-1,3-glucanase isozyme from Paenibacillus glycanilyticus FH11 in a new subgroup of family 87 α-1,3-glucanase, Journal of Bioscience and Bioengineering, 118(4) 378-385, 2014.10

    WasanaSuyotha, Shigekazu Yano, Takafumi Itoh, Hiroko Fujimoto, Takao Hibi, Takashi Tachiki, Mamoru Wakayama

    Multiple Authorship (Only Japanese)

  • Sensitive Enzymatic Method for Quantification of Theanine, Principal Umami Component in Commercial Tea Beverage, Food Science and Technology Research, 19(5) 909-913, 2013.10

    Yoichiro SHIMIZU, Masashi IMAOKA, Shigekazu YANO, Yosuke SAWARAGI, Kazuyoshi TAKAGI, Mamoru WAKAYAMA

    Multiple Authorship (Only Japanese)

  • Domain structure and function of α-1,3-glucanase from Bacillus circulans KA-304, an enzyme essential for degrading basidiomycete cell walls, Biosci Biotechnol Biochem., 77(3) 639-647, 2013.03

    24 Suyotha W, Yano S, Takagi K, Rattanakit-Chandet N, Tachiki T, Wakayama M

    Multiple Authorship (Only Japanese)

  • Functional analysis of the α-1,3-glucan synthase genes agsA and agsB in Aspergillus nidulans: agsB is the major α-1,3-glucan synthase in this fungus, PLoS One, 8(1) , 2013.01

    Yoshimi A, Sano M, Inaba A, Kokubun Y, Fujioka T, Mizutani O, Hagiwara D, Fujikawa T, Nishimura M, Yano S, Kasahara S, Shimizu K, Yamaguchi M, Kawakami K, Abe K.

    Multiple Authorship (Only Japanese)

  • Surface α-1,3-glucan facilitates fungal stealth infection by interfering with innate immunity in plants, PLoS Pathog., 8(8) , 2012.08

    Fujikawa T, Sakaguchi A, Nishizawa Y, Kouzai Y, Minami E, Yano S, Koga H, Meshi T, Nishimura M.

    Multiple Authorship (Only Japanese)

  • Production, purification and characterization of D-aspartate oxidase from the fungus Trichoderma harzianum SKW-36, Advances in Bioscience and Biotechnology, 3(1) 7-13, 2012.02

    21 Shigekazu Yano, Hiroyuki Ozaki, Shinji Matsuo, Masahiro Ito, Mamoru Wakayama、

    Multiple Authorship (Only Japanese)

  • Engineering the substrate specificity of Alcaligenes D-aminoacylase useful for the production of D-amino acids by optical resolution, Journal of Chromatography B, 879(29) 3247-3252, 2011.11

    Yano S, Haruta H, Ikeda T, Kikuchi T, Murakami M, Moriguchi M, Wakayama M.

    Multiple Authorship (Only Japanese)

  • Expression in Escherichia coli of a gene encoding type II L-asparaginase from Bacillus subtilis, and characterization of its unique properties, Annals of Microbiology, 61(3) 517-524, 2011.09

    Onishi Y, Yano S, Thongsanit J, Takagi K, Yoshimune K, Wakayama M.

    Multiple Authorship (Only Japanese)

  • N-Terminal Region of Chitinase I of Bacillus circulans KA-304 Contained New Chitin-Biding Domain, Bioscience, Biotechnology, and Biochemistry, 75(2) 299-304, 2011.02

    Yano S, Suyotha W, Honda A, Takagi K, Rattanakit-Chandet N, Wakayama M, Tachiki T.

    Multiple Authorship (Including Foreigners)

  • Molecular cloning and characterization of γ-glutamyltranspeptidase from Pseudomonas nitroreducens IFO12694, Bioscience, Biotechnology, and Biochemistry, 74(9) 1936-1939, 2010.09

    Imaoka M, Yano S, Okumura M, Hibi T, Wakayama M

    Multiple Authorship (Only Japanese)

  • Identification of glutaminase- producing lactic acid bacteria isolated from Nham, a traditional Thai fermented food and characterisation of glutaminase activity of isolated Weissella cibaria, Annals of Microbiology, 59(4) 671-675, 2009.12

    Thongsanit J, Tanikawa M, Yano S, Tachiki T, Wakayama M

    Multiple Authorship (Only Japanese)

  • Dynamics of cell wall components of Magnaporthe grisea during infectious structure development, Molecular Microbiology, 70(4) 553-570, 2009.08

    Fujikawa T, Kuga Y, Yano S, Yoshimi A, Tachiki T, Abe K, Nishimura M

    Multiple Authorship (Only Japanese)

  • 「Role of chitin binding domain of chitinase A of Streptomyces cyaneus SP-27 in protoplast formation from Schizophyllum commune, Bioscience, Biotechnology, and Biochemistry, 73(3) 733-735, 2009.03

    Yano S, Honda A, Rattanakit-Chandet N, Noda Y, Wakayama M, Plikomol A, Tachiki T.

    Multiple Authorship (Including Foreigners)

  • Overexpression of type I L-asparaginase of Bacillus subtilis in Escherichia coli, rapid purification and characterization of recombinant type I L-asparaginase, Annals of Microbiology, 58(4) 711-716, 2008.12

    Yano S, Minato R, Thongsanit J, Tachiki T. Wakayama M.

    Multiple Authorship (Only Japanese)

  • Identification of a glutaminase- producing bacterial strain isolated from Thai fermented pork sausage and characterisation of glutaminase production, Annals of Microbiology, 58(4) 671-675, 2008.12

    Thongsanit J, Yano S, Tachiki T, Wakayama M

    Multiple Authorship (Only Japanese)

  • Cloning and expression of chitinase A gene from Streptomyces cyaneus SP-27: the enzyme participates in protoplast formation of Schizophyllum commune, Bioscience, Biotechnology, and Biochemistry, 72(7) 1853-1859, 2008.07

    Yano S, Honda A, Rattanakit N, Noda Y, Wakayama M, Plikomol A, Tachiki T

    Multiple Authorship (Including Foreigners)

  • Purification and characterization of chitinase A of Streptomyces cyaneus SP-27: an enzyme participates in protoplast formation from Schizophyllum commune mycelia, Bioscience, Biotechnology, and Biochemistry, 72(1) 54-61, 2008.01

    Yano S, Rattanakit N, Honda A, Noda Y, Wakayama M, Plikomol A, Tachiki T.

    Multiple Authorship (Including Foreigners)

  • Analysis of essential amino acid residues for catalytic activity of glutaminase from Micrococcus luteus K-3, Journal of Bioscience and Bioengineering, 102(4) 362-364, 2007.10

    Yano S, Kamemura A, Yoshimune K, Moriguchi M, Yamamoto S, Tachiki T, Wakayama M.

    Multiple Authorship (Only Japanese)

  • Purification of Aspergillus sp. S1-13 chitinases and their role in saccharification of chitin in mash of solid-state culture with shellfish waste, Journal of Bioscience and Bioengineering, 103(6) 535-541, 2007.06

    Rattanakit N, Yano S, Plikomol A, Wakayama M, Tachiki T

    Multiple Authorship (Including Foreigners)

  • Characterization of salt-tolerant glutaminase from Stenotrophomonas maltophilia NYW-81 and its application in Japanese soy sauce, Journal of Industrial Microbiology and Biotechnology, 32(9) 383-390, 2006.09

    Wakayama M, Yamagata T, Kamemura A, Bootim N, Yano S, Tachiki T, Yoshimune K, Moriguchi M.

    Multiple Authorship (Including Foreigners)

  • Cloning and expression of an α-1,3-glucanase gene from Bacillus circulans KA-304: the enzyme participates in protoplast formation of Schizophyllum commune, Bioscience, Biotechnology, and Biochemistry, 70(7) 1754-1763, 2006.07

    Yano S, Wakayama M, Tachiki T.

    Multiple Authorship (Including Foreigners)

  • Cloning and Expression of a Bacillus circulans KA-304 gene Encoding Chitinase Ⅰ,which Participates in Protoplast Formation of Schizophyllum commune, Bioscience, Biotechnology, and Biochemistry, 69(3) 602-609, 2005.03

    Yano S, Rattanakit N, Wakayama M, Tachiki T.

    Multiple Authorship (Including Foreigners)

  • A Chitinase Indispensable for Formation of Protoplast of Schizophyllum commune in Basidiomycete-lytic Enzyme Preparation Produced by Bacillus circulans KA-304, Bioscience, Biotechnology, and Biochemistry, 68(6) 1299-1305, 2004.06

    Yano S, Rattanakit N, Wakayama M, Tachiki T

    Multiple Authorship (Including Foreigners)

  • Occurrence of a specific protein in basidiomycete-lytic enzyme preparation produced by Bacillus circulans KA-304 inductively with a cell-wall preparation of Schizophyllum commune, Bioscience, Biotechnology, and Biochemistry, 67(9) 1976-1982, 2003.09

    Yano S, Yamamoto S, Toge T, Wakayama M, Tachiki T.

    Multiple Authorship (Including Foreigners)

  • Saccharification of Chitin Using Solid-State Culture of Aspergillus sp. S1-13 with Shellfish Waste as a Substrate, Journal of Bioscience and Bioengineering, 95(4) 391-396, 2003.04

    Rattanakit N, Yano S, Wakayama M, Tachiki T.

    Multiple Authorship (Including Foreigners)

  • Utilization of Shrimp Shellfish Waste as a Substrate for Solid-State Cultivation of Aspergillus sp. S1-13: Evaluation of a Culture Based on Chitinase Formation Which is Necessary for Chitin-Assimilation, Journal of Bioscience and Bioengineering, 93(6) 550-555, 2002.06

    Rattanakit N, Plikomol A, Yano S, Wakayama M, Tachiki T.

    Multiple Authorship (Including Foreigners)

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Review Papers

  • α-1,3-Glucanase: present situation and prospect of research, World Journal of Microbiology and Biotechnology, 32(3) , 2016.02

    Wasana Suyotha, Shigekazu Yano, Mamoru Wakayama

Grant-in-Aid for Scientific Research

  • Grant-in-Aid for Scientific Research(B),2023.04 - 2024.03

  • Grant-in-Aid for Scientific Research(C),2022.04 - 2025.03

  • Grant-in-Aid for Scientific Research(C),2017.04 - 2020.03

Other external funds procured

  • Transglycosylation activity of alpha-1,3-glucanase Agl-KA under semi-dry condition,2017.04 - 2020.03,Transglycosylation activity of alpha-1,3-glucanase Agl-KA under semi-dry condition

    α-1,3-Glucanase Agl-KA from Bacillus circulans KA-304 hydrolyzes the α-1,3-glucoside linkages in α-1,3-glucan. In this study, we investigated transglycosylation activity of Agl-KA under a low water content. Lyophilized Agl-KA and nigero-oligosaccharides were stored at 80% humidity. After incubation, transglycosylation activity was confirmed, and the optimum pH of the transferring reaction was 10.
    We also analyzed molecular structure of the catalytic domain of Agl-KA. The structure was determined using X-ray crystallography. The catalytic unit consists of a complex structure of two tandemly connected regions, the N-terminal galactose-binding-like region and the C-terminal right-handed β-helix region. Biochemical assays have found that Asp1067, Asp1090, and Asp1091 are important for catalysis, and these residues indeed locate to the putative substrate binding cleft, which forms a closed end and explains the product specificity.

    Japan Society for the Promotion of Science